Maine Dil Tujhko Diya is the story of romance between a simple, down-to-earth guy Ajay (Sohail Khan) and a beautiful, vulnerable girl Ayesha (Sameera Reddy).
Ayesha is the elder daughter of a rich business tycoon Mr. Verma (Kabir Bedi), an overprotective father who has kept his two daughters away from the influences of modern world.
Ayesha goes to study in college for her graduation and comes in contact with Ajay who is the leader of a group called Aryans, responsible for handling the security in college. Ajay's soft-spoken nature and his humble, down-to-earth attitude soon makes Ayesha his ardent admirer. Ajay also helps Ayesha find her identity besides being just her father's daughter.
Ajay and Ayesha's friendship becomes an eyesore for Mr. Chopra (Dilip Tahil), business partner of Ayesha's father. Chopra is a cunning man who harbors the dream of lapping all of Verma's fortunes by marrying his son to Ayesha.
Chopra begins belittling Ajay in Verma's eyes. He also hires the Mumbai's don Bhaijaan (Sanjay Dutt) to get rid of Ajay. Luckily Ajay escapes Bhaijaan's clutches as it is the month of Ramazaan, and Bhaijaan, being a religious man, wont hurt a soul in this pious month.
Still, Chopra succeeds in his nefarious plans and gets Ajay beaten and thrown out of the city. In turn Ajay vows that he will marry Ayesha within 24 hours.
What happens next? Will Ajay overcome all the hurdles to win Ayesha's hand? Will Bhaijaan have a change of heart and help Ajay prove his worth to Ayesha's adamant father?